PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Abreu C.O., Martinez A.C., Moraes W., Juvenal J.C. & Moreira N. 2009. [Reproductive characteristics of Brazilian dwarf brocket deer (Mazama nana).] Características reprodutivas de veado-bororó-do-sul ou veado-mão-curta (Mazama nana). Pesquisa Veterinária Brasileira 29(12):993-998. Departamento de Medicina Veterinária, Universidade Estadual de Maringá, Campus Umuarama, Estrada da Paca s/n, Maringá, PR 87506-370. E-mail: cassianabreu@gmail.com Mazama nana is the least known of Brazilian deer species. The male reproductive parameters are still unknown, but apparently they did not show reproductive seasonality. In this work were used nine males of M. nana in reproductive age, kept in captivity. They were assessed for weight, height of wither, crown-rump length, the situation of horns, testicular volume and consistency. The sperm was collected by electro-ejaculation and subjected to analyses of motility, vigor and morphology. The correlation between age, weight, height of wither, crown-rump length, testicular volume and characteristics of the ejaculate (volume, motility, vigor and sperm concentration) were evaluated by the procedure Corr., of SAS®. The mean ± standard deviation observed for weight (kg), crown-rump length (cm) and height of wither (cm) were: 15.72±1.98, 74.9±3.05, and 48.5±2.06 respectively. For primary reproductive parameters were observed: volume of ejaculate (91.46±68.24ìl); motility (70±8.16%); vigor (3.0±0.67); sperm concentration (1536x106 ±351x106 spermatozoa per ml). For sperm morphology was found a high percentage of abnormal cells (40.90%), with predominant defects in the tail (25.95%).

• Veado-bororó-do-sul veado-mão-curta Mazama nana

Abstract in Portuguese:

RESUMO.- Abreu C.O., Martinez A.C., Moraes W., Juvenal J.C. & Moreira N. 2009. [Reproductive characteristics of Brazilian dwarf brocket deer (Mazama nana).] Características reprodutivas de veado-bororó-do-sul ou veado-mão-curta (Mazama nana). Pesquisa Veterinária Brasileira 29(12):993-998. Departamento de Medicina Veterinária, Universidade Estadual de Maringá, Campus Umuarama, Estrada da Paca s/n, Maringá, PR 87506-370. E-mail: cassianabreu@gmail.com Dos cervídeos brasileiros, a espécie Mazama nana é a menos conhecida. Os parâmetros reprodutivos para os machos ainda são desconhecidos, mas parece que não apresentam sazonalidade reprodutiva. Neste trabalho foram utilizados nove machos de Mazama nana em idade reprodutiva, mantidos em cativeiro. Foram avaliados quanto ao peso corporal, altura de cernelha, comprimento crânio-caudal, situação dos chifres, volume e consistência testicular. O sêmen foi colhido por eletroejaculação e submetido a análises de motilidade, vigor e morfologia espermática. As correlações entre idade, peso, comprimento crânio-caudal, altura de cernelha, volume testicular e características do ejaculado (volume, motilidade, vigor e concentração do sêmen) foram avaliadas pelo procedimento Corr., do SAS®. As médias ± desvio padrão observados para peso (kg), comprimento crânio-caudal (cm) e altura de cernelha (cm) foram: 15,72±1,98, 74,9±3,05 e 48,5±2,06, respectivamente. Em relação aos parâmetros reprodutivos primários foram observados: volume do ejaculado (91,46±68,24µl); motilidade (70±8,16%); vigor (3,0±0,67); concentração espermática (1536x106 ±351x106 espermatozóides por ml). Em relação à morfologia espermática, foi observada uma alta porcentagem de células anormais (40,90%), sendo predominante os defeitos de cauda (25,95%).

Abstract in English:

ABSTRACT.- Juvenal J.C., Erdmann R.H., Moreira N., Moraes W., Cubas P.H., Delgado L.E.S., Carvalho A.L. & Pachaly J.R. 2008. [Chemical restraint of tigrinas, Leopardus tigrinus, for semen collection with allometrically scaled doses of tiletamine, zolazepam, and xylazine.] Contenção farmacológica de gato-do-mato-pequeno, Leopardus tigrinus, para colheita de sêmen, pela associação de tiletamina zolazepam e xilazina. Pesquisa Veterinária Brasileira 28(11):541-546. Curso de Medicina Veterinária, Universidade Pa-ranaense, Cx. Postal 162, Umuarama, PR 87502-970, Brazil. E-mail: pachaly@uol.com.br This paper reports the anesthetic effects of the combination of tiletamine HCl, zolazepam HCl, and xylazine HCl in tigrinas, Leopardus tigrinus Schreber, 1775 (Fam. Felidae), submitted to semen collection by electroejaculation. Three different protocols and the individual anesthetic doses were calculated by interspecific allometric scaling, based on the usual recommendations for a 10.0 kg domestic dog: On Protocol 1 (n=10) the basis for calculation was 5.0mg/kg for tiletamine + zolazepam and 0.5mg/kg for xylazine; on Protocol 2 (n=12) 5.0mg/kg for tiletamine + zolazepam and 0.75mg/kg for xylazine; and on Protocol 3 (n=11) 5.0mg/kg for tiletamine + zolazepam and 1.0mg/kg for xylazine. The tigrinas were anesthetized on three different occasions with a minimum interval of 30 days. During 120 minutes after the drug administration cardiac and respiratory frequencies, rectal temperature, limb myorelaxation and sensitivity to deep pain were monitored. Latency period, anesthetic period, and contamination of the semen with urine were also monitored. From a total of 32 collections, 10 samples (31.2%) and 18 aliquots (0.07%) were contaminated and rejected, but this episodes were not detrimental for semen analysis and processing. A discrete increase in rectal temperature during electroejaculation caused by muscle contraction, followed by temperature decrease, was observed. Cardiac and respiratory frequency varied during the experiment, but remained within physiological standards for the species. The three tested protocols showed to be safe and effective to produce analgesia and anesthesia in L. tigrinus during semen collection by electroejaculation.

• Chemical restraint anesthesia tigrina Leopardus tigrinus allometric scaling semen collection

Abstract in Portuguese:

ABSTRACT.- Juvenal J.C., Erdmann R.H., Moreira N., Moraes W., Cubas P.H., Delgado L.E.S., Carvalho A.L. & Pachaly J.R. 2008. [Chemical restraint of tigrinas, Leopardus tigrinus, for semen collection with allometrically scaled doses of tiletamine, zolazepam, and xylazine.] Contenção farmacológica de gato-do-mato-pequeno, Leopardus tigrinus, para colheita de sêmen, pela associação de tiletamina zolazepam e xilazina. Pesquisa Veterinária Brasileira 28(11):541-546. Curso de Medicina Veterinária, Universidade Pa-ranaense, Cx. Postal 162, Umuarama, PR 87502-970, Brazil. E-mail: pachaly@uol.com.br This paper reports the anesthetic effects of the combination of tiletamine HCl, zolazepam HCl, and xylazine HCl in tigrinas, Leopardus tigrinus Schreber, 1775 (Fam. Felidae), submitted to semen collection by electroejaculation. Three different protocols and the individual anesthetic doses were calculated by interspecific allometric scaling, based on the usual recommendations for a 10.0 kg domestic dog: On Protocol 1 (n=10) the basis for calculation was 5.0mg/kg for tiletamine + zolazepam and 0.5mg/kg for xylazine; on Protocol 2 (n=12) 5.0mg/kg for tiletamine + zolazepam and 0.75mg/kg for xylazine; and on Protocol 3 (n=11) 5.0mg/kg for tiletamine + zolazepam and 1.0mg/kg for xylazine. The tigrinas were anesthetized on three different occasions with a minimum interval of 30 days. During 120 minutes after the drug administration cardiac and respiratory frequencies, rectal temperature, limb myorelaxation and sensitivity to deep pain were monitored. Latency period, anesthetic period, and contamination of the semen with urine were also monitored. From a total of 32 collections, 10 samples (31.2%) and 18 aliquots (0.07%) were contaminated and rejected, but this episodes were not detrimental for semen analysis and processing. A discrete increase in rectal temperature during electroejaculation caused by muscle contraction, followed by temperature decrease, was observed. Cardiac and respiratory frequency varied during the experiment, but remained within physiological standards for the species. The three tested protocols showed to be safe and effective to produce analgesia and anesthesia in L. tigrinus during semen collection by electroejaculation.